Last but not least, Geneious Prime is the perfect tool to have on your Mac if you need to easily insert DNA fragments into a vector with matching overhangs, to ligate multiple fragments with matching overhangs, to simulate restriction cloning, InFusion Cloning and Gibson Assembly, as well as conversional PCR and TOPO cloning. In addition, with the help of Geneious Prime you can highlight plasmids using terminators, common promoters, restriction sites, cloning sites, affinity tags, reporter genes, replication origins, open reading frames and selectable marker genes. Comprehensive biochemistry analysis tool with advanced molecular cloning Also, thanks to the PlasMapper you can automatically annotate plasmid maps and expression vectors. Moreover, Geneious Prime can help you design primers, simulate PCR and test cloning strategies using smart tools for Gibson and restriction cloning. You can even read arbitrarily large documents like NGS reads or contig assemblies. Hence, you can assemble multiple combinations of Sanger, 454, SOLiD and Illumina data at the same time. Furthermore, Geneious Prime is capable to handle data from various types of sequencing machines with reads of different lengths, including paired-reads and mixed reads from different sequencing machines. The versatile Geneious Assemble is designed to manage read errors caused by incorrect bases or short indels. User-oriented graphical interface and trustworthy reference mapping On top of that, you can enjoy the eye-catching genome browser, sequence assembly and reference mapping. Regardless of your data type, you can use Geneious Prime to quickly assemble it using fine-tuned and advanced algorithms. What is more, you can display phylogeny along with the alignment and link back to a tree, and use the highlighting feature to quickly locate disagreements. You can also view annotations on the alignments, remove or insert gaps, strip columns, fix bad calls and join sequences. Thanks to the visual sequence alignment and editing you can effortlessly choose the desired algorithms to precisely align sequences without working with multiple files. Intuitive and easy-to-use sequence editing and analysis tools :: MORE INFORMATION Posted on 8 8 Categories DNA / Genome Analysis, Plasmid / Chemical Drawing Tags Analysis, DNA Cloning, Gene Construction Kit, Plasmid Mapping, Vector Manipulation Leave a comment on Gene Construction Kit 4.Geneious Prime is a well-designed and powerful sequence alignment, assembly and analysis application for genomics, microbiology, virology, infectious disease, synthetic biology, ecology, conservation genetics, population genetics, evolutionary biology, agriculture, as well as crop and plant science. The Gene Construction Kit software is available for both Windows and Macintosh users, and files can be shared across platforms allowing for easy collaboration. This DNA analysis software allows multiple files to be opened and displayed simultaneously, allowing DNA sequences to easily be copied and pasted between plasmids and vectors to represent real-world DNA cloning protocols. GCK eliminates tedious examination of DNA sequence data by automatically identifying open reading frames (ORF’s), keeping track of sticky ends during cutting and pasting of restriction enzyme digestion fragments, assisting with PCR primer design, and enabling comprehensive annotation of DNA sequence features. MACAW, PHYLIP, MacVector, Signal Scan, RasMol, Tree View and Strider. GCK allows easy manipulation of DNA sequences, either graphically or as sequence text – quickly saving users both time and money. The book contains two chapters about plasmid mapping, PCR design and sequencing. The Gene Construction Kit®(GCK) program has been the preferred plasmid mapping software of leading researchers for more than 20 years. :: MORE INFORMATION Posted on 0 0 Categories DNA / Genome Analysis Tags DNA Cloning, Sequence Analysis, SERIAL CLONER, Visualisation Leave a comment on SERIAL CLONER 2.6.1 – DNA Cloning, Sequence Analysis & Visualisation Gene Construction Kit 4.5 – Plasmid Mapping, DNA Cloning Analysis, Vector Manipulation Serial Cloner handles Annotations and Features both in the sequence and in the Graphic Map. Powerful graphical display tools and simple interfaces help the analysis and construction steps in a very intuitive way. Import from MacVector is also possible now. Serial Cloner also import files saved in the Vector NTI, ApE, pDRAW32 and GenBank formats. Serial Cloner reads and write DNA Strider-compatible files and import and export files in the universal FASTA format. Serial Cloner has been developed to provide a light molecular biology software to both Macintosh and Windows users. The DNA sequence was analyzed using MacVector and Glimmer software to identify potential protein coding regions in the plasmid.
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